Zfp697 is an RNA-binding protein that regulates skeletal muscle inflammation and remodeling

To investigate the molecular mechanisms governing the transition of skeletal muscle from atrophy to compensatory regeneration and hypertrophy, we employed a mouse model involving hindlimb unloading and subsequent reloading, conducting a comprehensive analysis of global gene expression using RNA-sequencing (RNA-seq). Gastrocnemius muscle samples were obtained from three groups: control mice, mice subjected to 10 days of hindlimb unloading-induced muscle atrophy, and mice reintroduced to normal cage activity for 1 day following the unloading period (reloading). Comparative gene expression profiling analysis of RNA-seq data for mouse gastrocnemius muscle from model of hindlimb unloading/reloading assessing transitions from atrophy to compensatory regeneration and hypertrophy. Control mice were kept in conventional cages throughout the entire protocol. Experiment was performed in replicates (Control n equals 4, Unloading n equals 2, Reloading n equals 3). Paired-end DNA libraries were prepared using Illumina TrueSeq and sequenced on Illumina HiSeq2000.