File S1 - Microbial Community Structure in Lake and Wetland Sediments from a High Arctic Polar Desert Revealed by Targeted Transcriptomics

Tables S1–S3, Photographs S1 and S2, Figures S1–S5. Table S1A. Basic water chemistry for the wetland. Table S1B. Basic water chemistry for the lake. Table S2. Forward and Reverse PCR primers used for each target sequence. Table S3. p-values for SH-like comparison. Photograph S1. Picture of Char Lake. Photograph S2. Picture of the wetland. Figure S1. Summary of the experimental procedure, including RNA treatments and quality controls. Figure S2. Cumulative bacterial clonal frequency of phyla identified using 16S rRNA clone libraries. Bottom panel details the most abundant phylum. Figure S3. Cumulative archaeal clonal frequency of phyla identified using 16S rRNA clone libraries. Bottom panel details the most abundant class. Figure S4. Amino acid alignment of selected lake and wetland sequences showing the PKDKVKP conserved motif for the lake sequences. Figure S5. Left and bottom view of the McrA protein in a model lake sequence (A, B), Methanospirillum hungatei (C,D) and a model wetland sequence (E,F). Region highlighted in blue represents the insert present in lake sequences that is modified and much shortened in wetland sequences. A comparable insert exists in the Methanospirillum hungatei McrA protein.
(PDF)