Natural product profiling training set

A collection of morphological fingerprints generated using the Cell Painting protocol with 4 different fluorophores. Probes used were Hoechst (DNA), concanavalin A (golgi), mitotracker (active mitochondria), and phalloidin/WGA (membrane and ER). Cells were treated with a library of ~12,500 different bioactive small molecules, and incubated for 48 hours (full methods in manuscript document). Plates were imaged using an EVOS M7000 microscope, and Cell Profiler (v4.2.1) was used to quantify morphological characteristics/features. Training set fingerprints are compiled here as raw data (.Rdata files for R import), and are used in the GitHub repository to create a database and normalize/QC/process data and train models.