Daily Patterns of Retinal and Choroidal Gene Expression and Circadian Biology in Myopia Pathogenesis

We investigated diurnal changes in gene expression in the retina and choroid at the onset of experimental myopia. We induced visual form deprivation myopia in young chicks, and isolated retinal and choroidal tissues separately in form- deprived and contralateral control eyes every 4 hours over a single 24-hour period. After applying RNA-Seq separately to each tissue extract, we used various bioinformatics analyses and gene expression profiling analyses to assess the retinas and choroids of experimental and control eyes at each time point.