RNA-seq in mouse WT and ABAT cKO T cells

Purpose: To gain more mechanistic insights into the effects of ABAT deletion on T cells, we performed RNAseq in activated CD4 WT and ABAT cKO TH0 cells. Methods: Total RNA was isolated according to the RNeasy Mini Kit (Qiagen) and treated with DNase I. RNA quality was assessed using the Agilent 2100 Bioanalyzer and RNA Nano Chip kit (Agilent Technologies, CA). RNA-seq libraries were generated using TruSeq Stranded Total RNA with Ribo-Zero Globin Complete kit (Illumina, CA). Approximately 60–80 million paired-end 150 bp reads were generated per sample using Illumina HiSeq 4000 platform Results: RNA-seq revealed an decreased pro-inflammatory gene signature in ABAT cKO T cells after activation Conclusions: Inhibition of ABAT suppresses TH17 but enhances iTreg cell differentiation mRNA profiles of activated WT and ABAT cKO T cells were generated by deep sequencing