Gut microbiota and Parabacteroides transcriptome related to intestinal barrier in older adults

This study aimed to investigate the relationship between intestinal barrier function, gut microbiota composition, and bacterial gene expression in older adults. Fecal 16S rRNA gene sequencing was performed to characterize gut microbial profiles, and RNA-seq analysis of Parabacteroides merdae cultures was conducted to elucidate transcriptomic responses to compound addition. The integrated data provide insights into microbial and host factors contributing to intestinal barrier integrity in aging.