Supplemental Material for Magwanga et al., 2018

Supplementary Fig. S1: Phylogenetic tree, gene
structure and motif compositions of LEA2
genes in upland cotton. The phylogenetic tree was constructed using MEGA 7.0.
Exon/intron structures of LEA genes
in upland cotton, exons introns and up/down-stream were represented by yellow
boxes, black lines and blue boxes, respectively. Protein motif analysis
represented by different colours, and each motif represented by number
Supplementary
Fig. S2:
conserved motifs in upland cotton, G.
hirsutum LEA2 proteins. The conserved motifs were obtained using MEME
program. The color scheme of the logo indicates amino acid types. Polar:
green=uncharged; blue=+vely charged; red=-vely charged; Non-polar:
violet/purple=aliphatic. As described by Dure, 2001; Supplementary Fig. S3: Alignment of the LEA2 protein sequences of Upland
cotton, Gossypium hirsutum. Amino
acid sequences were aligned using the ClustalW2 algorithm. Dashes indicate gaps
introduced for optimal alignment. The typical LEA2 sequence elements are bound
in boxes in different colours: K segment–red box; Y segment–green; S
segment–black; E segment–gray; R segment–purple; D segment–yellow
Supplementary Fig. S4. LEA2 genes distribution in A and D
cotton chromosomes: Chromosomal position of each LEA2 genes was mapped according to the upland cotton genome. The chromosome number is indicated at the
top of each chromosome
Supplementary Fig. S5: LEA2 genes distribution in upland cotton
chromosomes. Chromosomal position of each
LEA2 genes was mapped according to the upland cotton genome. The chromosome number is indicated at the
top of each chromosome
Supplementary
Table S1: List of primers used for upland cotton, G. hirsutum LEA2 genes
expression analysis under drought stress
Supplementary
Table S2: Conserved domain analysis of LEA2s proteins identified from
upland cotton using CDD tool from NCBI
Supplementary
Table S3: Transmembrane domains in LEA2s identified from upland cotton,
using TMHMM and SOSUI servers. ExpAA: The expected number of amino acids in
transmembrane helices. First60: The expected number of amino acids in
transmembrane helices in the first 60 amino acids of the protein. MP: Membrane
protein; TMH: The number of predicted transmembrane helices; Supplementary Table S4: Subcellular
location as determined by TargetP and Pprowler prediction tools. SP-secretory
pathways; C-chloroplast;-any other location; Supplementary Table S5:
Cis element analysis of putative LEA 2 promoters related to drought stress
Supplementary Table S6: LEA2 genes and miRNA targets