Costa et al. Multiple mating rescues offspring sex ratio but not productivity in a haplodiploid exposed to developmental heat stress

Summary: The data and scripts presented were used to assess how a sublethal high temperature (36°C) affects the reproductive system of a haplodiploid species. Using spider mites (<em>Tetranychus urticae</em>) developed either at 25°C or 36°C, we paired individuals in four treatments (♀25°C x <strong>♂</strong>25°C, ♀25°C x <strong>♂</strong>36°C, ♀36°C x <strong>♂</strong>25°C, ♀36°C x <strong>♂</strong>36°C), to measure the effect of temperature on the fertility of both sexes, mating and remating eagerness, and the paternity of the offspring of females with different mating histories. <br> Files available: We provide 3 .R files (Script_statistical_analysis_Fertility_and_Paternity_Share, Script_statistical_analysis_Mating_Behavior, Script_figures) and 6 .csv files (Dataset_Fertility_and_Paternity_share, Dataset_altered_Fertility_and_Paternity_share, Dataset_means_Fertility_and_Paternity_share, Dataset_Mating_behavior, Dataset_means1_Mating_behavior, Dataset_means2_Mating_behavior). <br> Script_statistical_analysis_Fertility_and_Paternity_Share This file contains the script used to make the statistical analysis for the Fertility and Paternity Share experiment. <br> Script_statistical_analysis_Mating_Behavior This file contains the script used to make the statistical analysis for the Mating Behavior experiment. <br> Script_figures This file contains the script used to create all the figures. <br> Dataset_Fertility_and_Paternity_share This file contains the raw data of the effect of temperature on fertility and paternity share. Table headers are explained below: <em>Temp_fxm_1M</em> - developmental temperatures of the female and male paired (1^st mating of the female). <em>Temp_fem</em> - developmental temperature of the female (25°C or 36°C). <em>Temp_male</em> - developmental temperature of the male (25°C or 36°C). <em>Type_M</em> - mating history of the female (SM, if single mated; DM, if double mated; MM, if multiple mated). <em>Rep</em> - replicate number per treatment. <em>Block</em> - day in which matings were observed. <em>Rtemp</em> - temperature of the observation room. <em>Rhum</em> - humidity of the observation room. <em>Fecundity</em> - number of eggs laid during 4 days per female. <em>Unhatched_eggs</em> - number of eggs that did not develop. <em>Total_daughters</em> - number of adult daughters counted. <em>Total_sons</em> - number of adult sons counted. <em>Total_contribution_males</em> - sum of adult daughters and unhatched eggs. <em>Total_offspring</em> - sum of adult daughters, adult sons and unhatched eggs. <em>Prop_daughters_ue</em> - proportion of adult daughters in the fertilized offspring (<em>Total_daughters</em> / <em>Total_contribution_males</em>). <em>Prop_fert_off</em> - proportion of adult daughters and unhatched eggs in the offspring (<em>Total_contribution_males</em> / <em>Total_offspring</em>). <em>Observations</em> - any observations made in each replicate. <br> Dataset_altered_Fertility_and_Paternity_share This file contains the same raw data as Dataset_Fertility_and_Paternity_share, and includes 4 more lines (in SM from each treatment) to deal with the lack of variance in <em>Total_daughters and Unhatched_eggs</em>, which impeded the statistical analysis. Table headers are similar to the ones in Dataset_Fertility_and_Paternity_share. <br> Dataset_means_Fertility_and_Paternity_share This file contains the means of each variable studied for the effect of temperature on fertility and paternity share. Table headers are explained below: <em>Temp_fxm_1M</em> - developmental temperatures of the female and male paired (1^st mating of the female). <em>Temp_fem</em> - developmental temperature of the female (25°C or 36°C). <em>Temp_male</em> - developmental temperature of the first male (25°C or 36°C). <em>Type_M</em> - mating history of the female (SM, if single mated; DM, if double mated; MM, if multiple mated). <em>Mean_prop_fem_ue</em> - mean proportion of daughters and unhatched eggs in the offspring per treatment and female mating history. <em>Mean_total_off </em>- mean number of offspring (daughters, sons and unhatched eggs) per treatment and female mating history. <em>Mean_prop_fem </em>- mean proportion of adult daughters in the fertilized offspring (daughters and unhatched eggs) per treatment and female mating history. <br> Dataset_Mating_behavior This file contains the raw data of the effect of temperature on mating behavior. Table headers are explained below: <em>Temp_fxm_1M</em> - developmental temperatures of the female and male paired (1^st mating of the female). <em>Temp_fem</em> - developmental temperature of the female (25°C or 36°C). <em>Temp_male</em> - developmental temperature of the male (25°C or 36°C). <em>Type_M</em> - mating history of the female (SM, if single mated; MM, if multiple mated). <em>Rep</em> - replicate number per treatment. <em>Block</em> - day in which matings were observed. <em>Rtemp</em> - temperature of the observation room. <em>Rhum</em> - humidity of the observation room. <em>Mating_rate</em> - number of times the female mated. <em>Lat_sec1</em> - latency to the first copulation of the female. <em>CopYN</em> - if the female mated a first time (1=yes, 0=no). <em>Cop_sec1</em> - duration of the first copulation of the female. <em>Lat_sec2</em> - latency to the second copulation of the female. <em>Cop2YN</em> - if the female mated a second time (1=yes, 0=no). <em>Cop_sec2</em> - duration of the second copulation of the female. <em>Fecundity</em> - number of eggs laid during 4 days per female. <em>Total_daughters</em> - number of adult daughters counted. <em>Total_sons</em> - number of adult sons counted. <em>Sex_ratio</em> - proportion of adult daughters in the offspring (adult daughters and sons). <em>Total_adults</em> - sum of adult daughters and sons. <em>Observations</em> - any observations made in each replicate. <br> Dataset_means1_Mating_behavior This file contains the means of each variable studied for the effect of temperature on mating behavior. Table headers are explained below: <em>Temp_fxm_1M</em> - developmental temperatures of the female and male paired (1^st mating of the female). <em>Temp_fem</em> - developmental temperature of the female (25°C or 36°C). <em>Temp_male</em> - developmental temperature of the first male (25°C or 36°C). <em>Mean_lat1</em> - mean latency to the first copulation of the female per treatment. <em>Mean_cop1</em> - mean duration of the first copulation of the female per treatment. <em>Mean_lat2</em> - mean latency to the second copulation of the female per treatment. <em>Mean_cop2</em> - mean duration of the second copulation of the female per treatment. <br> Dataset_means2_Mating_behavior This file contains the means of each variable studied for the effect of temperature on mating behavior present in the supplementary material. Table headers are explained below: <em>Temp_fxm_1M</em> - developmental temperatures of the female and male paired (1^st mating of the female). <em>Temp_fem</em> - developmental temperature of the female (25°C or 36°C). <em>Temp_male</em> - developmental temperature of the first male (25°C or 36°C). <em>Type_M</em> - mating history of the female (SM, if single mated; MM, if multiple mated). <em>Mean_off </em>- mean number of offspring (adult daughters and sons) per treatment and female mating history. <em>Mean_sexratio </em>- mean proportion of adult daughters in the offspring (adult daughters and sons) per treatment and female mating history.