NFAT5 induction by the tumor microenvironment enforces CD8 T cell exhaustion

To decipher if NFAT5 controls CD8 T cell exhaustion during chronic infection on a molecular level, we performed scRNA sequencing on sorted P14 WT and NFAT5 KO CD8 from the spleen of a chronic LCMV-infected mouse. Vb5 transgenic mice (CD45.1) were adoptively transferred with NFAT5 KO or wild-type (WT) P14 CD8 T cells from naive mice (CD45.2) and infected LCMV clone 13 (cl13). Total P14 cells were flow sorted from the spleen on day 28 post infection and processed for scRNAseq analysis.