Single cell transcriptomics of human abdominal aortic aneurysm [scRNA-seq]

An abdominal aortic aneurysm (AAA) is a pathological widening of the aortic wall characterized by loss of AoSMCs, extracellular matrix degradation and local inflammation. This condition is often asymptomatic until rupture occurs, leading to high morbidity and mortality rates. We conducted single-cell RNA sequencing (scRNA-seq) from AAA patients´s specimens to profile gene expression at single cell level and to gain insight on cell types relevant to disease dynamics. Overall design: To profile single-cell transcriptome and analyze the diversity of cell types present in human AAA tissue specimens, we carried out scRNA-seq from 4 AAA patients. Sequencing reactions were perfomed in duplicate.