多组学评估人类辅助生殖技术后代的表观基因组学的影响

为了阐明可归因于ARTs的表观遗传风险，我们通过亚硫酸氢盐还原表达测序（RRBS）、染色质免疫沉淀测序（ChIPseq）对137份脐带血（UCB）和158份亲代外周血（PPB）样本的DNA甲基组、33份UCB样本的组蛋白修饰（H3K4me3、H3K4me1、H3K27me3和H3K27ac）和32份UCB样品的转录组进行了分析，和RNA测序（RNA-seq）To clarify the epigenetic risks attributable to Assisted Reproductive Technologies (ARTs), we conducted multi-omic analyses of clinical samples: Reduced Representation Bisulfite Sequencing (RRBS) was used to profile the DNA methylomes of 137 Umbilical Cord Blood (UCB) samples and 158 Parental Peripheral Blood (PPB) samples; Chromatin Immunoprecipitation Sequencing (ChIP-seq) was applied to characterize the histone modifications (H3K4me3, H3K4me1, H3K27me3, and H3K27ac) of 33 UCB samples; and RNA Sequencing (RNA-seq) was utilized to analyze the transcriptomes of 32 UCB samples.

搜集汇总

背景与挑战

背景概述

该数据集通过多组学方法研究人类辅助生殖技术对后代表观基因组的影响，涵盖了DNA甲基化、组蛋白修饰和转录组分析。数据来源于人类脐带血和父母外周血样本，包含甲基化、组蛋白和转录组三个层面的分析数据，旨在揭示辅助生殖技术可能引起的表观遗传风险。