Snapshots of serine protease catalysis: (D) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 jumped to pH 10 for 2 minutes
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Snapshots of serine protease catalysis: (D) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 jumped to pH 10 for 2 minutes Descriptor: CALCIUM ION, ELASTASE 1, SULFATE ION Authors: Wilmouth, R.C, Edman, K, Neutze, R, Wright, P.A, Clifton, I.J, Schneider, T.R, Schofield, C.J, Hajdu, J. Deposit date: 2001-04-10 Release date: 2001-08-02 Last modified: 2024-11-13 Method: X-RAY DIFFRACTION (2.05 Å) Cite: X-Ray Snapshots of Serine Protease Catalysis Reveal a Tetrahedral Intermediate Nat.Struct.Biol., 8, 2001
丝氨酸蛋白酶(serine protease)催化反应快照:(D) 猪胰弹性蛋白酶(porcine pancreatic elastase)与人β-酪啡肽-7(human beta-casomorphin-7)之间形成的酰基酶中间体(acyl-enzyme intermediate)置于pH 10条件下反应2分钟。 描述符:钙离子(CALCIUM ION)、弹性蛋白酶1(ELASTASE 1)、硫酸根离子(SULFATE ION) 作者:Wilmouth, R.C、Edman, K、Neutze, R、Wright, P.A、Clifton, I.J、Schneider, T.R、Schofield, C.J、Hajdu, J 入库日期:2001-04-10 发布日期:2001-08-02 最后修改日期:2024-11-13 实验方法:X射线衍射(X-RAY DIFFRACTION),分辨率2.05埃(Å) 引用:《X射线快照揭示丝氨酸蛋白酶催化反应中的四面体中间体》,发表于《Nat.Struct.Biol.》,第8卷,2001年



