Gene expression profile at single cell level of hESC H9 derived hemognic niches at Day 18 of in vitro differentiation

Small drug (SB/CHIR) treatment and orbital shear treatment promotes the generation of hemogenic endothelium and multipotent hematopoieitic progenitor cells. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of hemogenic niches derived from hESC H9 line at day 18 of in vitro differentiation. Hemogenic niches derived from day 18 hESC H9 differentiation were harvested and anlyzed using scRNAseq. a 22 balanced experimental design was used to quantify the effect of circuilatory flow (shear) and small drug treatment (SB/CHIR).