Data underlying the manuscript: Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism

Summary

This
dataset underlies the figures and supplementary figures in the manuscript: “Lysates
of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal
FoxP3+RORγt+IL-17+ Tregs and improve metabolism”.

The
related study showed that exchanging the protein source in a high fat, high
sugar, westernised diet (WD) from casein to whole-cell lysates of the
non-commensal bacterium Methylococcus capsulatus Bath (McB) was sufficient to
reverse WD-induced changes in the gut microbiota to a state resembling that of
lean, low fat diet-fed mice, both under mild thermal stress (T22°C) and at
thermoneutrality (T30°C).

Type of data:
gut microbiota and cecal SCFA level changes due to change of the protein source
in a high fat, high sugar, westernised diet.

Subject of
data: Mus musculus

Population
characteristics: 6-7-week-old male C57BL/6JBomTac, C57BL/6JRj, C57BL/6N,
C57BL/6N-Rag2Tm1/CipheRj (RAG2-/-) mice acquired from Taconic Laboratories,
Denmark, or Janvier Labs, France.

Data

The raw data
underlying the figures and supplementary figures in the related manuscript are
shared openly here as .txt files in tab-separated value format. There are 94
files in total, and each is named in accordance with the particular figure and
subfigure it underlies. For more details on the particulars of each file, see
the figure captions in the related manuscript.

Corresponding author(s) for this study

Benjamin.
A. H. Jensen, Laboratory of Genomics and Molecular Biomedicine, Department of
Biology, Faculty of Science, University of Copenhagen, Denmark. [e-mail].

Karsten
Kristiansen, Address as above. [e-mail].

Tor
E. Lea , Faculty of Chemistry, Biotechnology and Food Science, Norwegian
University of Life Sciences, Norway. [e-mail].

Study approval

All experiments were
conducted in accordance with the EU directive 2010/63/EU as approved by the
Danish Animal Experiments Inspectorate (#2014-15-2934-01,027).