Transcriptome assay of maternal mRNA degradation in ERK-deleted oocytes

We analyzed the functions of ERK in maternal mRNA degradation in mouse oocytes. By comparing the degradation of transcripts in WT oocytes and KO oocytes, we are able to know the defects in maternal mRNA clearance in ERK-deleted oocytes, and identified the ERK target genes in oocyte maturation. a WT GV oocyte, a WT MII oocyte, a Erk-/- GV oocyte and a Erk-/- MII oocyte are performed RNA sequencing.我们针对细胞外调节蛋白激酶（ERK）在小鼠卵母细胞母源mRNA降解过程中的功能展开了研究。通过对比野生型（WT）与敲除型（KO）卵母细胞的转录本降解情况，我们得以明确ERK缺失卵母细胞在母源mRNA清除过程中的缺陷，并筛选鉴定出卵母细胞成熟过程中的ERK靶基因。本研究对野生型生发泡期（GV）卵母细胞、野生型第二次减数分裂中期（MII）卵母细胞、ERK纯合敲除GV卵母细胞以及ERK纯合敲除MII卵母细胞开展了RNA测序（RNA sequencing）。