GNPS - Photosynthetic metabolite used for nitrogen fixation

Using 13C bicarbonate labelling, metabolites from photosynthesis will be identified and depletion of metabolites after 1hour of photosynthesis inhibition will reveal metabolites used for nitrogen fixation. Bicarbonate will be added at the beginning of the day, and samples will be taken after 5hours. Cells will be treated with DCMU (photosynthesis inhibitor) at this 5h timepoint and left for an additional 1h incubation. Final samples will be taken at 6h (1 hour after DCMU addition) to assess depletion of the photosynthetic metabolites.

The work (proposal:https://doi.org/10.46936/10.25585/60008462) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.