Screening for H/ACA snoRNAs in Caenorhabditis elegans by GFP-FLAG-DKC-1 eCLIP.

To identify H/ACA snoRNAs, we generated a C. elegans strain overexpressing GFP-FLAG-DKC-1 and biochemically screened for GFP-FLAG-DKC-1-binding RNAs by enhanced crosslinking and immunoprecipitation (eCLIP) assay. Overall design: eCLIP studies were performed by Eclipse Bioinnovations Inc (San Diego, www.eclipsebio.com) according to the published single-end CLIP protocol.