Supporting data for “Discovery of Cell-Type-Specific mtDNA Common Deletion In Nasopharyngeal Carcinoma”

In early studies, a common deletion mutation in the mitochondrial genome (mtDNA-CD) was identified in nasopharyngeal carcinoma (NPC) patient samples. However, bulk sequencing protocols employed in past studies might hinder cancer-specific mutation detection, resulting in the heterogeneous results reported. Thus, we argue that the NPC samples should be examined at a single-cell resolution. In this study, we propose using scRNA-seq data to identify cell-type-specific deletion signatures in NPC samples. Since there is a lack of bioinformatics tools available for large-scale deletion detection, we developed a custom computational pipeline to detect deletion mutation at the single-cell level. In this study, we employed the use of nine publicly available scRNA-seq datasets for analysis.