Data on Pine Needle Extraction and Bioactivity Testing

The current study employed solid-liquid extraction using hexane to separate biomolecules from pine needles and GC/ MS QP2010 PLUS, Shimadzu for their identification. The solid and liquid cultures for microbial growth and UV-Vis to measure growth. Protein extraction was done using physical-chemical methods, separation using gel electrophoresis and analysis by MALDI-QUAD-TOF-MS-MS followed by Mascot software search for library comparison.

搜集汇总

背景与挑战

背景概述

该数据集记录了松针中生物分子的提取与生物活性测试过程，包括使用正己烷固液萃取、GC/MS鉴定、微生物培养及生长测量、蛋白质提取与凝胶电泳分离，以及MALDI-QUAD-TOF-MS-MS质谱分析结合Mascot库搜索。数据涵盖了从化学提取到生物测试的完整实验流程，适用于天然产物研究。