A pharmacophylogenetic study on two Artemisia species (Artemisia argyi and Artemisia indica) via multi-perspective analysis

Positive and Negative Ion metabolism datas of Artemisia argyi and Artemisia indica  at different stages (LC-MS) . A. argyi and A. indica were collected during the growing season of 2020 from an experimental field in Nanyang County (33°03’6.56” N, 112°49’36.91” E), Henan Province, China. A. argyi and A. indica leaves were obtained at three different growth stages: the early stage (April 6, 2020), middle stage (May 16, 2020), and later stage (June 26, 2020) .Freeze-dried leaves were crushed using a mixer mill for 1.5 min at 30 Hz. Then, 100 mg powder was extracted overnight at 4 °C using 1.0 mL 70% aqueous methanol. The extracts were filtered and analyzed using an ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) system (UPLC SHIMADZU Nexera X2; MS, Applied Biosystems 4500 Q TRAP). The analytical conditions were as follows: UPLC column, Agilent SB-C18 (1.8 µm, 2.1 mm×100 mm); solvent system, water (0.1% formic acid), acetonitrile (0.1% formic acid); gradient program, 95:5 v/v at 0 min, 5:95 v/v at 9.0 min, 5:95 v/v at 10.0 min, 95:5 v/v at 10.1 min, 95:5 v/v at 14 min; flow velocity, 0.35 mL/min; column oven, 40 °C; injection volume, 4 μL. The effluent was connected to an ESI-triple quadrupole-linear ion trap (QTRAP)-MS. The ESI source operation parameters were as follows: ion source, turbo spray; temperature, 550 °C; ion spray voltage, 5500 V/-4500 V; ion source gas I, gas II, and curtain gas were set at 50, 60 and 25.0 psi, respectively. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in the triple quadrupole (QQQ) and linear ion trap (LIT) modes, respectively. The declustering potential and collision energy for individual multiple reaction monitoring (MRM) transitions were determined. A specific set of MRM transitions was monitored for each period according to the metabolites eluted during this period. Metabolite analyses were performed according to the reported method.Based on the self-built database MWDB (Metware Biotechnology Co., Ltd. Wuhan, China), and a public database of metabolite information, MS was used for qualitative and quantitative analysis of the samples’ metabolites.