miR-200b is associated with cisplatin sensitivity in bladder cancer cells. Homo sapiens

We aimed to clarify the role of miR-200b in cisplatin (CDDP) sensitivity in bladder cancer (BCa). CDDP resistant T24 cells (T24RC) were transfected with a miR mimic negative control (NC) or a miR-200b mimic, after which cells were treated with or without CDDP. We found that ectopic miR-200b expression re-sensitized the T24RC cells to CDDP. Gene expression microarray analysis revealed that the combination of miR-200b and CDDP affected genes involved in CDDP sensitivity and cytotoxicity. Overall design: CDDP resistant T24 cells (T24RC) were transfected with a miR mimic negative control (NC) or a miR-200b mimic using Lipofectamine RNAiMAX and incubated for 72 h. Transfectants were then treated with or without 1.6 μg/ml of CDDP for additional 72 h. Total RNA was extracted using a TRI Reagent.