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Infection of bone marrow derived macrophages with M. tuberculosis CDC1551

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE35362
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I infected 8 flasks of resting murine bone marrow derived macrophages with M. tuberculosis CDC1551. Individual samples of intracellular Mtb were collected at 2hr, and days 2,4,6,8,10,12, and 14 post-infection using the GTC (guanidine thiocyanate) method as previously published. A 2hr "no macrophage" control was used as a common denominator in the two-color hybridizations. I have two independent biological replicates of this experiment. Groups of assays that are related as part of a time series. Time: culture time post-infection time_series_design
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2012-07-12
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