Screening of Lactic Acid Bacteria with Low Antibiotic Resistance Genes and Its Effects on Silage Quality and Resistance Genes

This study aims to screen lactic acid bacteria (LAB) with low antibiotic resistance genes (ARGs) and evaluate their effects on silage quality and ARG dissemination. By isolating LAB from natural sources (e.g., silage, fermented feed) and combining whole-genome sequencing with PCR-based ARG profiling, low-ARG strains are selected. Subsequent silage fermentation experiments assess their impact on nutritional preservation (crude protein, water-soluble carbohydrates), fermentation parameters (pH, lactic acid content), and microbial community dynamics. Metagenomic sequencing or qPCR is employed to compare ARG abundance in silages inoculated with low-ARG LAB versus conventional strains, evaluating horizontal gene transfer risks. The research expects to establish a safe LAB strain library for silage inoculation, clarify ARG transfer mechanisms, and propose practical strategies to optimize silage production while minimizing ARG spread. By aligning agricultural productivity with the "One Health" initiative, this study seeks to mitigate ARG transmission from feed to livestock and the environment.