Zfr regulates ADAR-mediated RNA editing in mouse primary neurons

We used RNA-seq of mouse primary cortical neurons transfected with control shRNAs or shRNAs targeting ADAR1, ADAR2, or Zfr to test for A-to-I RNA editing level differences between control and knockdown neurons. To determine RNA editing sites controlled by ADAR1 and ADAR2, we compared editing levels in two biological replicates of mouse primary neurons transfected with shControl#1, shADAR1, and shADAR2. shADAR2 biological replicate 1 has three technical replicates, which are different RNA-seq libraries prepared from RNA extracted from the same neurons. We compared editing levels between shControl#1 and shADAR1 and shADAR2 to determine which editing sites are regulated by each ADAR. We compared editing levels between shControl#2 and shZfr to determine which editing sites were regulated by Zfr.