Time series of Escherichia coli BL21(DE3) after temperature upshift from 37°C to 42°C, tiling arrays. Time series of Escherichia coli BL21(DE3) after temperature upshift from 37°C to 42°C, tiling arrays

Heat-responsive and time-resolved changes in transcriptome of E. coli BL21(DE3) Experimentally mapped transcriptome structure of Escherichia coli BL21(DE3) by hybridizing total RNA (including RNA species <200 nt) to genome-wide high-density tiling arrays (60 mer probes tiled every 10 nt). Overall design: One sample was taken from chemostat at 37°C (time point of -30min) and eight time-series samples were taken from chemostat at 42°C (time points of 2min, 10min, 30min, 1h, 2h, 4h, 8h, and 40h)