Gene expression analysis of human cancer associated fibroblasts (CAFs) stimulated with Platelet-Derived Growth Factor (PDGF)-CC

This study is part of a larger effort set to determine the factors requried for the crosstalk between tumor cells and fibroblasts in breast cancer. Specifically, the primary aim of this microarray analysis is to detail the soluble factors secreted by cancer-asociated fibroblasts following stmulation with PDGF-CC. CAF2 cells were either left untreated (control) or stimulated with human recombinant PDGF-C for 6 or 48 hours. Three (3) biological replicates for each of the three conditions were used for the analysis, for a total of 12 samples