Structural data used for morphology based prediction of neuronal functional types (Boulanger-Weill et al, 2025 - https://www.biorxiv.org/content/10.1101/2025.03.14.643363v2)

Zebrafish Hindbrain Neuron Structural Data Structural data for morphology-based prediction of neuronal functional types in the zebrafish hindbrain, as described in Boulanger-Weill, Kämpf et al. (2025). The dataset contains 563 neurons across three imaging modalities - photoactivation (PA), correlative light-electron microscopy (CLEM), and electron microscopy (EM) - with SWC skeleton tracings, 3D meshes, synapse annotations, and functional type labels. These data support an LDA classifier that predicts four functional cell types from 13 morphological features with 83.6% cross-validation accuracy. Contents File Description metadata.xlsx Cell inventory with functional labels, training flags, and cell identifiers (3 sheets: PA, CLEM, EM) paGFP.zip 47 photoactivation neurons - SWC skeletons and functional dynamics clem_zfish1.zip 301 CLEM neurons - registered SWC skeletons, OBJ meshes, synapse locations em_zfish1.zip 215 EM neurons - registered SWC skeletons and meshes After downloading, unzip into a single directory so the layout is: CLEM_paper_data/ metadata.xlsx paGFP/ clem_zfish1/ em_zfish1/ Coordinate System All coordinates are registered to the Z-Brain atlas reference frame (Randlett et al., 2015). Units are microns. SWC node type labels follow standard conventions: Label Structure 1 Soma 2 Axon 3 Dendrite 4 Presynapse 5 Postsynapse Functional Types Abbreviation Full name iMI Motion integrator, ipsilateral cMI Motion integrator, contralateral MON Motion onset SMI Slow motion integrator Quick Start git clone https://github.com/jboulanger91/Zebrafish_CLEM.git cd "Zebrafish_CLEM/4. Morphology_based_prediction_of_neuronal_functional_types" pip install -r requirements.txt python scripts/setup_data_paths.py python functional_type_prediction/classifier_prediction/pipelines/pipeline_main.py Code & Documentation Classification pipeline: jboulanger91/Zebrafish_CLEM / 4. Morphology_based_prediction_of_neuronal_functional_types Parent repository (registration, connectivity, modeling): jboulanger91/Zebrafish_CLEM EM connectome explorer: Neuroglancer (requires Gmail login)