Supplementary Material for: The Role of Angiopoietins in Cardiovascular Outcomes of Kidney Transplant Recipients- An Ancillary Study From the FAVORIT

Introduction: Kidney transplant recipients (KTRs) have increased risk of cardiovascular disease (CVD) mortality. We investigated vascular biomarkers, angiopoietin-1 and angiopoietin-2 (angpt-1, -2), in CVD development in KTRs.

Methods: This ancillary study from the FAVORIT, evaluates the associations of baseline plasma angpt-1,-2 levels in CVD development (primary outcome) and graft failure (GF) and death (secondary outcomes) in 2000 deceased donor KTRs. We used Cox regression to analyze the association of biomarker quartiles with outcomes. We adjusted for demographic, CVD and transplant-related variables; medications; urine albumin-to-creatinine ratio and randomization status. We calculated areas under the curves (AUC) to predict CVD or death, and GF or death by incorporating biomarkers alongside clinical variables.

Results: Participants’ median age was 52 IQR [45, 59] years: with 37% women and 73% identifying as white. Median time from transplantation was 3.99 IQR [1.58, 7.93] years and to CVD development was 2.54 IQR [1.11-3.80] years. Quartiles of angpt-1 were not associated with outcomes. Whereas higher levels of angpt-2 (quartile 4) were associated with about 2 times the risk of CVD, GF and death [aHR 1.85 (1.25 - 2.73), P

引言：肾脏移植受者（KTRs）心血管疾病（CVD）死亡率风险增加。本研究旨在探讨KTRs心血管疾病发展中的血管生物标志物——血管生成素-1和血管生成素-2（angpt-1，-2）的作用。

方法：本辅助研究来自FAVORIT研究，评估了2000名已故供体肾脏移植受者在心血管疾病发展（主要结局）和移植物衰竭（GF）及死亡（次要结局）中基线血浆angpt-1,-2水平的相关性。我们采用Cox回归分析生物标志物四分位数与结局之间的关联。我们在调整人口统计学、心血管疾病和移植相关变量、药物、尿液白蛋白-肌酐比和随机化状态后，计算曲线下面积（AUC）以预测心血管疾病或死亡，以及GF或死亡，通过结合生物标志物和临床变量。

结果：参与者的中位年龄为52岁（四分位距 [45, 59]），其中37%为女性，73%自认为是白人。移植至发生心血管疾病的中位时间为3.99年（四分位距 [1.58, 7.93]），而自移植至心血管疾病发展的中位时间为2.54年（四分位距 [1.11-3.80]）。angpt-1的四分位数与结局无关联。然而，angpt-2水平（第四四分位数）较高者与心血管疾病、移植物衰竭及死亡的风险增加约2倍相关[aHR 1.85 (1.25 - 2.73)，P