Homo sapiens Single-cell Transcriptome

Single-cell sequencing of human peripheral blood mononuclear cells (PBMC) isolated from 10 healthy control individuals, 14 sporadic Parkinson (PD) individuals, 3 multiple system atrophy (MSA) individuals, and 3 progressive supranuclear palsy (PSP) individuals. Samples were age and sex-matched, and 5-plexed before library was prepared according to 10X Genomics 5' v1.1 kit recommendations. In parallel to the transcriptome libraries (GEX), libraries of T-cell receptor variable regions (VDJ) were prepared for the same cells. Both libraries were processed with the Chromium Next GEM Chip G lanes prior to sequencing with a NovaSeq 6000 S4 (Illumina) sequencer, which generated 6 mixed fastq per library.