Amplicon sequencing of genomically-encoded guide RNA sequences for the Brunello CRISPR library in eHap cells. Amplicon sequencing of genomically-encoded guide RNA sequences for the Brunello CRISPR library in eHap cells

eHap CRISPR-Cas9 mutagenized cells with no selection serves as a control. RPS6p antibody staining on the mutagenized eHap cells was used in a flow-sorting approach to isolate RPS6p high and low populations respectively. The genomic DNA from these different samples was isolated, subjected to PCR to amplify the genomically-encoded guide RNA sequences, the product(s) of which was then sequenced on a Hi-Seq X Ten instrument by Novogene.