CDK12 regulates alternative last exon mRNA splicing and promotes invasion of a breast cancer cell line

We performed mRNA sequencing on 184-hTert and SK-BR-3 and MDA-MB-231 cell lines treated with siRNA directed to CDK12 (Cyclin dependent kinase 12) or a scrambled siRNA control. For each cell line, we generated 6 RNA-seq libraries (3 treatment replicates and 3 control replicates) to study the differential expression and the differential splicing patterns regulated by CDK12. Overall design: Examination of differential mRNA abundance and splicing patterns between the CDK12 siRNA treated samples and control samples for SK-BR-3 and 184-hTert and MDA-MB-231 cell lines.