Chloroplast precursor protein overaccumulation triggers multilevel reprogramming and a heat shock-like response in plant cells [Ribo-Seq]

Most of chloroplast proteins are encoded by nucleus ,synthesied in cytosol as precursors. theses precursor contains tranzit peptide and will be cut off after import finished. How plant response to chloroplast precursor overaccumulation stress(cPOS) is still unkown. Here we constructed constutitive and induced overexpression line to lead preClpD accumulation. Proteomic analysis revealed cytosolic ribosome upregulated, which means translation changed upon cPOS. So we conducted RNA-seq and Ribo-seq experiment to find which genes response at transcription level or translation level.Finally, we want to uncover the cellular events upon chloroplast precursor overaccumulation. Overall design: we conducted costitutive ClpD overexpression line and western blot showed preClpD accumulated in 6-day-old seedlings. polysome profiling showed that polysome increase upon cPOS.So we used this plant materials to conduct RNA-seq and Ribo-seq to find transcription-dependent expressed genes and translation-dependent expressed genes.