Identification of the casual gene for the Ms2 locus in onion

In addition to the previously reported restorer-of-fertility (Rf) locus, Ms, a novel Rf locus was identified in this study from onion populations in which the Ms locus was fixed as a homozygous recessive genotype. A combined approach of bulked segregant analysis and RNA-seq was used to identify a chromosomal location of the novel Rf locus and to develop tightly linked molecular markers.