Genome-scale CRISPR-Cas9 screen of Wnt/ß-catenin signalling identifies therapeutic targets for Colorectal Cancer (RNA-seq)

To identify novel regulators and therapeutic targets of ß-catenin-driven colorectal cancer development, genome-scale CRISPR-cas9 screens of Wnt/ß-catenin were conducted and identified KMT2A as a key player of ß-catenin-driven CRC progression. RNA-seq data was integrated with CHIP-seq and other experiments to assess the effect of KMT2A knockout on the transcriptomic profiling of CRC cells. Overall design: 1) RNA samples from triplicates of NTC sgRNA-targeting DLD1 cells; 2) RNA samples from triplicates of two different KMT2A sgRNA-targeting DLD1 cells