Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: Sanger Protocol Development - Mock

The HMP early mock pilot represents the dideoxy sequencing of 16S rRNA genes amplified from the early even Mock community (constructed by Broad). These experiments were conducted using a variety of pre-production versions of the sanger sequencing protocol to test primers, buffers, and PCR conditions.