Phosphorylation status of AKT and mTOR by immunohistochemistry.

1(+) Increased (or equivalent) staining relative to normal mucosa (−) decreased staining.2Nonuniform staining throughout the tumors.3AKT-T308 is significantly downregulated in intestinal adenocarcinomas relative to colonic adenomas,4AKT-S473 is significantly downregulated in intestinal adenocarcinomas relative to colonic adenomas, Fisher exact P5AKT-S473 is downregulated in drug treated intestinal tumors relative to untreated intestinal tumors (staining judged more or less intense relative to normal crypts) Fisher exact P = 0.0553.6mTOR significantly upregulated in adenocarcinomas of the small intestine relative to adenomas of the colon, Fisher exact P = 0.0013.7p-mTOR localizes subcellular to the nucleus in adenocarcinomas and the cytoplasm of adenomas, Fisher exact P = 0.0022.