ChIP-seq of maize transcription factor LOB30

Maize LOB30 (Zm00001d036435) is a transcription factor and is specifically expressed in anthers. Our previous RNA-seq data showed that expression of some genes were upregualted in maize lob30 mutant maize anthers. To confirm these genes are the downstrem target genes, we generated proLOB30: GFP-LOB30 transgenic maize lines, collected stage 9 to stage10 anther materials and performed ChIP-seq using the GFP antibody. ChIP-seq of maize transcription factor LOB30, transgenic maize anthers expressing the fused protein GFP-LOB30 driven by its native promoter were collected and crosslinked. The GFP antibody was used for chromatin immunoprecipitation.