Venetoclax cooperates with ionizing radiation to attenuate Diffuse Midline Glioma tumor growth

This article highlights the radiation induced cytotoxic effect of the BCL2 inhibitor, venetoclax, in diffuse midline gliomas (DMG). 1. RNA-seq: We performed bulk RNA sequencing of DMG cells exposed to 6Gy radiation and found the genes upregulated after radiation compared to unirradiated controls. 2. DNA Repair shRNA screen: We also performed a DNA Repair shRNA screen and identified the genes which are responsible for radioresistance in DMG. Overall design: 1. RNA-seq samples were obtained from SF8628 DIPG cells radiated at 6Gy and unirradiated controls. 2. DNA Repair shRNA samples obtained from SF8628 cells transduced with DNA Repair shRNA pool and then either radiated at 6Gy or not radiated. Genomic DNA was collected at Day 21 after radiation.