Deficiency of RPSA reduces binding of P65 to proinflammatory cytokines promoter regions in HSV-1 infection

After HSV-1 infection, ribosomal protein RPSA can recognize viral DNA and promotes the expression of proinflammatory cytokines. Through ChIP-seq, we found that the enrichment of P65 in proinflammatory cytokines(Il1b, Il6, Il12b, etc.) promoter regions was decreased due to Rpsa deficiency. Overall design: We constructed Rpsa induced konckout RAW264.7 cell lines and performed chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for P65 in WT and Rpsa-iKO RAW264.7 cells infected with or without HSV-1.