Gene expression analysis of hPSC-derived macrophages (iMACs) encountering Streptococcus pneumoniae.

Purpose: to analyze gene expression changes of iMACs encountering S. pneumoniae. Methods: SMART-seq2 amplified polyA RNA from sorted iMACs in the co-cultures. Results: the transcriptome of iMACs changed after encountering S. pneumoniae. Conclusions: the gene expression profiles of S. pneumoniae stimulated iMACs indicated iMACs undergoing inflammatory response and the immune response was distinct with iMACs encountering other pathogens such as HCV or SARS-CoV-2. Overall design: Perform RNA-seq of control iMACs, and iMACs stimulated by S. pneumoniae serotype 14.