RNA-seq analysis of miR-210 KO 293T cells

To study the effects of miR--210 on 293T cells and hypoxia response, we used the CRISPR/Cas9 system to knockout (KO) the human miR-210 gene in 293T cells. We then added DMOG to mimic hypoxia condition and analyzed the RNA expression profile of wildtype (WT) and KO cells under normal and hypoxic conditions. DMOG treatment and RNA-seq were performed three times (experiment 1, 2, and 3). Overall design: Total RNA was isolated after 48h treatment, and RNA-seq analysis was performed.