Transcriptome of the knockout mutant and control group of Synechococcus sp. PCC 7002 under 1.2 mol/L NaCl condition

This transcriptome data is part of a published study.Sample processing and quality control:Four groups of samples were collected: wild-type and mutant strains cultured in basal A⁺ medium for 24 h, and wild-type and mutant strains cultured in A⁺ medium containing 1.2 mol/L NaCl for 24 h (each group had three biological replicates). The initial A₇₃₀ was 0.02. All four groups were first cultivated in A⁺ medium with 0.3 mol/L NaCl for 4 days until the logarithmic growth phase. Then, one set of wild-type and mutant cultures was randomly selected and centrifuged at 6,000 rpm for 5 min. After discarding the supernatant, the pellets were resuspended in A⁺ medium containing 0.3 mol/L NaCl. The other set of wild-type and mutant cultures was centrifuged under the same conditions and resuspended in A⁺ medium containing 1.2 mol/L NaCl. After 24 h, the four groups of algal cells were collected by centrifugation at 12,000 rpm for 5 min. The supernatant was removed, and the samples were immediately flash‑frozen in liquid nitrogen and stored at −80°C until use. Total RNA was extracted, and RNA quality was assessed using a Nanodrop and Agilent 2100. Integrity was checked by 1.5% agarose gel electrophoresis. Samples meeting the requirements were used for library construction.Sequencing and data quality control:Libraries were sequenced on an Illumina PE150 platform. Raw reads were quality‑filtered using fastp software. The reference genome of Synechococcus sp. PCC 7002 was downloaded from the NCBI database (https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_000019485.1). Clean reads after quality control were accurately aligned to the reference genome using HISAT2 software. Read quantification was performed with the featureCounts function in the subread package. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) functional enrichment analyses of differentially expressed gene sets were carried out using the clusterProfile software (P < 0.05).