RNA-seq of Aphid Species: Myzus persicae, Myzus cerasi and Rhopalosiphum padi transcriptome. RNA-seq of Aphid Species: Myzus persicae, Myzus cerasi (taxid:93721) and Rhopalosiphum padi (taxid:40931) isolate:Myzus persicae genotypes O, J and F. Myzus cerasi, Rhopalosiphum padi | breed:Aphids | cultivar:Aphids were maintained in growth rooms at 18°C with a 16 hour light and 8 hour dark period. M. persicae (genotype O, F and J) was maintained on potato (Solanum tuberosum cv. Desiree), M. cerasi was maintained on American Land Cress (Barbarea verna) and R. padi was maintained on barley (Hordeum vulgare cv. Optic).

RNA sequencing of aphid dissected material was conducted and differential expression analysis between heads (contains salivary glands. Salivary glands are located in the heads and are thought to synthesise effector molecules) and bodies (without nymphs) was employed to identified transcripts which were upregulated in “heads”, those encoding signal peptides and no transmembrane domain were considered putative effectors.