HITI and INDEL analysis by next-generation sequencing
收藏NIAID Data Ecosystem2026-03-13 收录
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资源简介：
SpCas9 INDEL frequency and HITI was evaluated at the on-target site of guide RNA targeting either the mouse or pig rhodopsin by next-generation sequencing. Overall design: Two gRNA-treated samples of either mouse or pig were used to amplify the gRNA on-target site with forward primer that anneals to the mRho/pRho sequence and two reverse primers, one that anneals to mRho/pRho and the other to DsRed on the donor DNA.
创建时间：
2021-11-12
