Additional file 11 of Optimization of a deep mutational scanning workflow to improve quantification of mutation effects on protein–protein interactions
Macroautophagy is a highly conserved eukaryotic cellular pathway involving the engulfment of macromolecules, organelles, and invading microbes by a double-membrane compartment and subsequent lysosomal
Protein hotspot residues are key sites that mediate protein-protein interactions. Accurate identification of these residues is essential for understanding the mechanism from protein to function and fo
(A) RT-PCR analysis of calpains 8 and 9 mRNA in the WT, Capn8+/−, Capn8−/−, Capn9+/−, and Capn9−/− gastric mucosa using primer pairs: calpain 8-5′/-3′ for calpain 8, calpain 9-5′/-3′ for calpain 9, an
Shown is a Coomassie stained gel after SDS-PAGE analysis of a pull-down experiment of His-tagged IFT70 or SUMO-tagged IFT88 with 3 different truncated GST-tagged IFT52 constructs.This experiment reve