p53 inhibitor iASPP is an unexpected suppressor of KRAS and inflammation driven pancreatic cancer

To investigate the transcriptomic effects of iASPP deletion or p53 mutation in the KRASG12D background Pdx1-Cre was used to delete iASPP in oncogenic KRASG12D expressing (KC) background to generate KC;iASPPΔ8/Δ8 mice. KC and KC;iASPPΔ8/Δ8 mice were then crossed with mice containing a p53R172H allele (equivalent to human p53 hotspot mutation R175H) to generate KPC and KPC;iASPPΔ8/Δ8 cohorts. Primary cell cultures were then derived from pancreatic ductal adenocarcinoma tissue for RNA-seq. Comparative gene expression profiling analysis of RNA-seq data for KC, KC;iASPPΔ8/Δ8,KC, and KC;iASPPΔ8/Δ8 mouse cell lines.