Transcriptome sequencing of spongy moth's larvae and pupa

The spongy moth (Lymantria dispar Linnaeus, Lepidoptera: Erebidae) stands as a pervasive international threat, marked by its designation as one of the "world's 100 worst invasive species" by the International Union for Conservation of Nature (IUCN), owing to its voracious leaf-eating habits encompassing over 500 plant species. Its strong flight ability facilitates its spread and invasion. The present study aims to elucidate the expression of genes related to spongy moth's wing development, utilizing the Illumina Novaseq6000 sequencing platform for comprehensive transcriptome sequencing and bioinformatic analysis of total RNA extracted from larvae and pupae. The study focuses on specimens collected from Shuozhou city (SZ) in Shanxi province and Zunyi city (ZY) in Guizhou province. Results revealed pivotal processes of protein functional structure conformation, transport, and signal transduction in functional gene annotation during the two developmental stages of spongy moth.