5-Hydroxymethylcytosine profiling of aging hippocampi

We profiled 5-hydroxymethylcytosine patterns in hippocampi using hydroxymethylated DNA immunoprecipitation followed by Illumina deep sequencing (hMeDIP-Seq). We profiled the hippocampi of 4 young (3 month old) and 4 aged (18 month old) C57BL/6 mice. Additionally, we profiled the 5-hmC patterns of hippocampi that were stereotactically injected with lentiviral constructs that overexpressed Tet2 or luciferase. Overall design: Examination of 5-hydroxymethylcytosine in hippocampi of two ages (3 months and 18 months) and Tet2 overexpression and control hippocampi