Modulation of the mouse liver epigenome following TCPOBOP exposure

Changes in the activating histone-H3 chromatin marks K4me1, K4me3, and K27ac were assayed in male mouse liver following exposure to TCPOBOP, an agonist ligand of the nuclear receptor CAR (constitutive androstane receptor). This work is part of a larger study, entitled: "Widespread Epigenetic Changes to the Enhancer Landscape of Mouse Liver Induced by a Specific Xenobiotic Agonist Ligand of the Nuclear Receptor CAR" (Tox Sci, 2019). Overall design: Chromatin immunoprecipitation (ChIP) analysis of histone marks was carried out using sonicated liver chromatin, either prepared by sonication of cross-linked liver nuclei, or by sonication of cross-linked whole liver tissue. A set of 6 individual liver chromatin samples (3 vehicle-treated, 3 TCPO-treated) was used for ChIP analysis of all three chromatin marks at 3 h; and a set of 7 other individual liver chromatin samples (3 vehicle-treated, 4 TCPOBOP-treated) was used for ChIP analysis of all three chromatin marks at 27 h, namely, H3K4me1, H3K4me3, and H3K27ac.