Coexpression and purification of HTT and HTT-HAP40 with NEDD4L and HAP1 2019/05/02

<strong>Project: </strong>Biophysical investigation of purified HTT protein and protein complex samples <strong>Experiment: </strong>Coexpression and purification of HTT and HTT-HAP40 with NEDD4L and HAP1 <strong>Date completed:­ </strong>2019/05/02 <strong>Rationale: </strong>HAP1 and NEDD4L have been suggested as high probability interaction partners of HTT although it is unclear if they interact with HTT or HTT-HAP40. Coexpression of the constructs was conducted in baculoviral expression system (Sf9 cells) to determine if they copurify with HTT or HTT-HAP40.